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KMID : 1147720150080060294
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Journal of Acupuncture and Meridian Studies
2015 Volume.8 No. 6 p.294 ~ p.300
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A Novel Technique for Visualizing the Intralymphatic Primo Vascular System by Using Hollow Gold Nanospheres
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Carlson Eric
Perez-Abadia Gustavo
Adams Staci
Zhang Jin Z.
Kang Kyung-A.
Maldonado Claudio
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Abstract
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Until recently, the primo vascular system (PVS) has been unnoticed by most anatomists due to the small diameter and translucent features of the threadlike network. These properties make primo vessels (PVs) difficult to visualize for harvest and for further characterization. One particular PVS subtype that is located within the lymphatic vessels (LVs) is of strong interest because with a proper contrast, these long PVs can be visualized through the transparent LV wall and can be harvested to provide sufficient sample material for analysis. The most common method to visualize this PVS subtype utilizes Alcian blue as the contrast agent. This technique is effective, but tedious, and has relatively low repeatability. The purpose of this study was to develop a new technique that allows reliable visualization of the intralymphatic PVS (IL-PVS) in a user-friendly manner. The method was designed to provide optical contrast to the PVS by taking advantage of the porous nature of the PV's external wall and interstitial matrix. Turquoise-green-colored hollow gold nanospheres (HGNs) in the size range of 50?125 nm were found to provide excellent optical contrast for the IL-PVS in rats. The PVS was visualized within 10 minutes after HGN administration at a 95% success rate.
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KEYWORD
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hollow gold nanospheres, lymph nodes, lymphatics, microscopy, optical imaging, primo vascular system, primo vessel
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